4 ECTS credits
100 h study time
Offer 1 with catalog number 4017592ENR for all students in the 1st semester
at
a (E) Master - advanced level.
- Semester
- 1st semester
- Enrollment based on exam contract
- Impossible
- Grading method
- Grading (scale from 0 to 20)
- Can retake in second session
- Yes
- Enrollment Requirements
- Following courses are mutually exclusive: 'High Throughput Techniques (4 ECTS)", "High Throughput Techniques (6 ECTS-credits)", "Biomed. Engineering techniques", "Comb. Chem. and Drug Design" and "Comb. Biochem. and Drug Design". Ook studenten van de master biomedische wetenschappen en de master Biomedical Research kunnen dit vak opnemen. Voor hen geldt enkel de voorwaarde dat ze ingeschreven moeten zijn in de master BMW/master biomedical Reserach. Voor masterstudenten Bio-ingenieurswetenschappen geldt volgende voorwaarde: geslaagd zijn voor Gentechnologie, Microbiologie, Organische chemie: reactiviteit, Organische chemie: structuur, Proteïnechemie, functie en structuur.
- Taught in
- Faculty
- Faculty of Sciences and Bioengineering Sciences
- Department
- Bio-Engineering Sciences
- Educational team
- Jo Van Ginderachter
Nick Devoogdt
Steven Ballet
(course titular)
- Activities and contact hours
- 26 contact hours Lecture
- Course Content
Part Combinatorial Chemistry
The next topics are discussed:
- Solution Phase Combinatorial Synthesis: Parallel Synthesis, One-pot Synthesis, Scavengers, Quenchers, Resin bound reagents, Solution Phase Combinatorial Chemistry using Dendrimers, Classical purification by extraction, Fluorous phase chemistry.
- Solid Phase Combinatorial Synthesis: Resins, Linkers, Solution Phase Synthesis versus Solid Phase Synthesis.
- Mixed versus Parallel Synthesis,
- Identification of the Active Component (Mix-and-split Synthesis, Micro Analysis, Off-bead Mass Spectrometry, On-bead Mass Spectrometry, On-bead analysis with NMR-Spectroscopy, Infrared Spectrometry, Iterative Deconvolution, Recursive Method, Positional Scanning), Encoding of Libraries (Chemical Tagging, Binary Encoding, Radio Frequency (RF) Markers, Laser (optical) Encoding, Fluorescence Encoding),
- Dynamic combinatorial chemistry
- Varying case studies
Part of Combinatorial BIO-chemistry:
In this part we review:
- the construction and screening of genomic and cDNA banks.
- the selection of peptide libraries via phage-display, introducing M13 gene3 containing phasmids, selection procedures and providing solutions to remediate the reduced performance of virions.
- the alternative non-phage display systems and screenings, including in vitro selection systems such as SELEX, ribosome display, RNA peptide fusion and compartimentalised systems.
- the selection techniques for functional entities and in vitro transcription/translation and protein interactions on µ-arrays and the generation of new man-made proteins.
- Techniques to determine cellular characteristics via fluorescence (immunohistochemistry, fluorescence microscopy, flow cytometry)
- Mass cytometry of cells
- Techniques to determine the epigenome of cells (ATAC-seq, ChIP-seq)
- Techniques to determine the transcriptome of individual cells (single cell RNA-seq)
- Course material
- Course text (Required) : Partim Combinatoriële Chemie, Nota's college, powerpoint presentaties, cursustekst, Door de prof
Digital course material (Required) : Partim Combinatoriële BIO-chemie, Cursusnota's en powerpoint presentaties
- Additional info
N/A
- Learning Outcomes
-
General competences
Part Combinatorial Chemistry
The student
- understands the principles of combinatorial chemistry, both in solution and on solid phase, and their application in medicinal chemistry and the pharmaceutical industry .
- understands the chemical reactivity of the different conversions used during this course
- is acquainted with (high-throughput) techniques (synthetic and analytical) used in combinatorial chemistry and knows their advantages and disadvantages.
Part of Combinatorial BIO-chemistry
The student understands
- the various techniques used to construct immense large combinatorial libraries of bio-molecules
- their screening in a high-throughput approach for functional biochemical molecules
- the techniques to determine characteristics of individual cells, at the level of proteins, epigenome and transcriptome
- Grading
-
The final grade is composed based on the following categories:
Oral Exam determines 75% of the final mark.
Written Exam determines 25% of the final mark.
Within the Oral Exam category, the following assignments need to be completed:
- Combinatorial Chemistry
with a relative weight of 1
which comprises 25% of the final mark.
Note: Oral examination with written preparation.
- Combinatorial BIO-chemistry an
with a relative weight of 2
which comprises 50% of the final mark.
Note: Oral examination with written preparation.
The final marks are the average of the two parts,traking into account the number of hours foreseen for each subpart.
Within the Written Exam category, the following assignments need to be completed:
- Combinatorial Chemistry
with a relative weight of 1
which comprises 25% of the final mark.
Note: Written examination
- Additional info regarding evaluation
Part Combinatorial Chemistry (Prof S. Ballet)
Oral exam with written preparation and written exam. Knowledge and insight of the student are tested.
Part of Combinatorial BIO-chemistry (Prof Van Ginderachter/Devoogdt)
Oral examination with written preparation.
The final marks are determined as follows: Combinatorial Chemistry (Prof Ballet): 3/6; BIO-chemistry (Prof Devoogdt): 2/6; BIO-chemistry (Prof Van Ginderachter): 1/6.
If the student obtains at least half of the score for an individual part (Combinatorial chemistry or BIO-chemistry), partial marks are transferred to the second session and to the next academic year. Students may not relinquish partial marks.
- Allowed unsatisfactory mark
- The supplementary Teaching and Examination Regulations of your faculty stipulate whether an allowed unsatisfactory mark for this programme unit is permitted.
Academic context
This offer is part of the following study plans:
Master of Bioengineering Sciences: Cell and Gene Biotechnology: Medical Biotechnology (only offered in Dutch)
Master of Bioengineering Sciences: Cell and Gene Biotechnology: Molecular Biotechnology (only offered in Dutch)
Master of Bioengineering Sciences: Cell and Gene Biotechnology: Agrobiotechnology (only offered in Dutch)
Master of Bioengineering Sciences: Chemistry and Bioprocess Technology: Food Biotechnology (only offered in Dutch)
Master of Bioengineering Sciences: Chemistry and Bioprocess Technology: Chemical Biotechnology (only offered in Dutch)