5 ECTS credits
125 h study time
Offer 1 with catalog number 4017442FNR for all students in the 1st semester
at
a (F) Master - specialised level.
- Semester
- 1st semester
- Enrollment based on exam contract
- Impossible
- Grading method
- Grading (scale from 0 to 20)
- Can retake in second session
- Yes
- Taught in
- English
- Faculty
- Faculty of Sciences and Bioengineering Sciences
- Department
- Bio-Engineering Sciences
- Educational team
- Jo Van Ginderachter
Steven Ballet
(course titular)
Timo De Groof
- Activities and contact hours
- 26 contact hours Lecture
52 contact hours Independent or External Form of Study
- Course Content
Part Combinatorial Chemistry
The next topics are discussed:
- Solution Phase Combinatorial Synthesis: Parallel synthesis, One-pot synthesis, scavengers, quenchers, resin-bound reagents, solution phase combinatorial chemistry using dendrimers, classical purification by extraction, fluorous phase chemistry,
- Solid Phase Combinatorial Synthesis: Resins, linkers, solution phase synthesis versus solid phase synthesis.
- Mixed versus parallel synthesis,
- Identification of the active components (Mix-and-split synthesis, micro analysis, Off-bead mass spectrometry, On-bead mass spectrometry, On-bead analysis with NMR spectroscopy, infrared spectrometry, iterative deconvolution, recursive method, positional scanning), encoding of libraries (chemical tagging, binary encoding, radio frequency (RF) markers, laser (optical) encoding, fluorescence encoding),
- Dynamic combinatorial chemistry
- Varying case studies
Part of Combinatorial BIO-chemistry:
In this part we review:
- the construction and screening of genomic and cDNA banks.
- the selection of peptide libraries via phage-display, introducing M13 gene3 containing phasmids, selection procedures and providing solutions to remediate the reduced performance of virions.
- the alternative non-phage display systems and screenings, including in vitro selection systems such as SELEX, ribosome display, RNA peptide fusion and compartimentalised systems.
- the selection techniques for functional entities and in vitro transcription/translation and protein interactions on µ-arrays and the generation of new man-made proteins.
- Techniques to determine cellular characteristics via fluorescence (immunohistochemistry, fluorescence microscopy, flow cytometry)
- Mass cytometry of cells
- Techniques to determine the epigenome of cells (ATAC-seq, ChIP-seq)
- Techniques to determine the transcriptome of individual cells (single cell RNA-seq)
- Course material
- Digital course material (Required) : Combinatoriële biochemie, Notities en powerpoint presentaties
Course text (Required) : Combinatoriële chemie, Cursustekst, notities en powerpoint presentaties, Door de prof
Course text (Required) : Drug Design, Cursustekst, notities en powerpoint presentaties, Door de prof
- Additional info
N/A
- Learning Outcomes
-
General competencies
Part Combinatorial Chemistry
The student
- understands the principles of combinatorial chemistry, both in solution and on solid phase, and their application in medicinal chemistry and the pharmaceutical industry .
- understands the reactivity of the different conversions used during this course
- is acquainted with (high-throughput) techniques (synthetic and analytical) used in combinatorial chemistry and knows their advantages and disadvantages.
Part of Combinatorial BIO-chemistry
The student understands
- the various techniques used to construct immense large combinatorial libraries of bio-molecules
- their screening in a high-throughput approach for functional biochemical molecules
- the techniques to determine characteristics of individual cells, at the level of proteins, epigenome and transcriptome
- Grading
-
The final grade is composed based on the following categories:
Oral Exam determines 67% of the final mark.
Written Exam determines 33% of the final mark.
Within the Oral Exam category, the following assignments need to be completed:
- examen mondeling
with a relative weight of 2
which comprises 44.7% of the final mark.
Note: Partim Combinatoriële BIO-chemie
- examen mondeling
with a relative weight of 1
which comprises 22.3% of the final mark.
Note: Partim Combinatoriële Chemie
Within the Written Exam category, the following assignments need to be completed:
- examen schriftelijk
with a relative weight of 1
which comprises 33% of the final mark.
Note: Partim Combinatoriële Chemie
- Additional info regarding evaluation
Part Combinatorial Chemistry (Prof S. Ballet)
Oral exam with written preparation and written examination
Part of Combinatorial BIO-chemistry (Prof Van Ginderachter/Devoogdt)
Oral examination with written preparation.
The final marks are the average of the grades obtained for each of the three parts. The student must succeed (10/20) for all three parts in order to pass the complete course.
If the student obtains at least half of the score for an individual part, partial marks are transferred to the second session and to the next academic year. Students may not relinquish partial marks.
- Allowed unsatisfactory mark
- The supplementary Teaching and Examination Regulations of your faculty stipulate whether an allowed unsatisfactory mark for this programme unit is permitted.
Academic context
This offer is part of the following study plans:
Master of Molecular Biology: Standaard traject