5 ECTS credits
150 u studietijd

Aanbieding 1 met studiegidsnummer 1022196BNR voor alle studenten in het 1e semester met een verdiepend bachelor niveau.

Semester
1e semester
Inschrijving onder examencontract
Niet mogelijk
Beoordelingsvoet
Beoordeling (0 tot 20)
2e zittijd mogelijk
Ja
Onderwijstaal
Engels
Faculteit
Faculteit Ingenieurswetenschappen
Verantwoordelijke vakgroep
Bio-ingenieurswetenschappen
Onderwijsteam
Jo Van Ginderachter (titularis)
Kiavash Movahedi
Sophie De Buyl
Leonardus van Grunsven
Onderdelen en contacturen
30 contacturen Hoorcollege
30 contacturen Werkcolleges, practica en oefeningen
Inhoud

1) Introduction to Cell Biology

This part aims to introduce the students to the main basic principles of Cell Biology. The focus lies on the theoretical knowledge of these fundamental cell biology principles.

Following aspects of cell biology will be discussed:

  • Universal features of eukaryotic cells
  • The plasma membrane
  • The cytoskeleton
  • Compartmentalization of the cell: The nucleus, The endoplasmic reticulum, The Golgi apparatus, The mitochondrion

 

2) Important cell biology techniques and their applications

This part aims to introduce the students to 3 main cell biology techniques, which are amply used in research (both in academia as in industry). The focus lies on both the theoretical knowledge of these fundamental cell biology techniques and an understanding of their applications.

Following techniques will be discussed:

  • Manipulating DNA and proteins. This chapter includes: techniques for the fractionation of cell components, techniques for the isolation of proteins, techniques to isolate genes (PCR, reverse transcription, gene cloning, plasmid expression vectors, construction of transgenic mice)
  • Light microscopy. This chapter includes: light as electromagnetic waves, light refraction, basis of a compound microscope, introduction to bright-field microscopy, introduction to dark-field microscopy, introduction to fluorescence microscopy (basics of fluorescence, filter sets, photodetectors), using fluorescence to visualize cells or cellular structures (functional dyes, immunofluorescence, fluorescent proteins, FRAP, FLIP, FRET), introduction to laser scanning confocal microscopy, introduction to two-photon microscopy.
  • Flow cytometry and cell sorting. This chapter includes: the flow cytometric set-up, electrostatic cell sorting, compensation

 

3) Mathematical modelling of complex cell biology systems (Enzyme kinetics and biochemical reaction networks)

This part aims to introduce the students to mathematical modelling of exemplary biological problems. The focus lies on applicability, rather than theoretical knowledge.

Following aspects will be discussed:

  • Modelling chemical reaction networks (law of mass action, numerical simulations, separation of time scales and model reduction
  • Modelling biochemical reactions (enzyme kinetics)
  • Modelling gene regulatory networks (modelling gene expression)

 

4) Tissue techniques. This part aims to introduce the students to the principle techniques for studying and manipulating tissue.

The following aspects will be discussed:

  • Cell and tissue culture techniques
  • Histology and histological techniques
  • Electron microscopy
Bijkomende info

PowerPoint presentations discussed during the lectures and a list of exercises available in Canvas. The recommended handbook is 'Molecular Biology of the Cell (Alberts et al, Garland Science).

 

Leerresultaten

Leerresultaten

1. Understand the working principles of techniques to culture cells and tissues

2. Understanding of histology and histological techniques and being able to interpret histological coupes

3. Understand various quantitative techniques for the quantitative analysis of cell morphology, cell properties, structure and function and be able to apply quantitative analysis

4. Understand the relation between cell composition and cell function as inferred from the above-mentioned techniques

Beoordelingsinformatie

De beoordeling bestaat uit volgende opdrachtcategorieën:
Examen Andere bepaalt 100% van het eindcijfer

Binnen de categorie Examen Andere dient men volgende opdrachten af te werken:

  • Examen met een wegingsfactor 1 en aldus 100% van het totale eindcijfer.

Aanvullende info mbt evaluatie
Attendance to the practicals of the course are compulsory.
 
The students will be given (by drawing) questions from each part of the course (from Introduction to Cell Biology, Cell Biology Techniques, Mathematical Modeling and Tissue Biology Techniques).
The students will have approximately 1 hour for a written preparation (the written preparation will not be marked, it only serves to allow the students to reflect on the questions), followed by approximately 1 hour oral exam.
Each lecturer will interrogate the student about the question dealing with his/her part of the course and will provide a separate mark, which will only be based on the oral exam.
The final mark for Quantitative Cell Biology will be the average of the three marks given by the individual lecturers.
In case the student fails (overall average score <10/20), he/she will only have to retake the part(s) for which he/she failed.
Toegestane onvoldoende
Kijk in het aanvullend OER van je faculteit na of een toegestane onvoldoende mogelijk is voor dit opleidingsonderdeel.

Academische context

Deze aanbieding maakt deel uit van de volgende studieplannen:
Bachelor in de ingenieurswetenschappen: biomedische ingenieurstechnieken
Voorbereidingsprogramma Master of Science in Biomedical Engineering: International Students (enkel aangeboden in het Engels)