4 ECTS credits
120 u studietijd
Aanbieding 1 met studiegidsnummer 4023206ENR voor alle studenten in het 2e semester met een verdiepend master niveau.
• General introduction to OMICS technologies
• Mass spectrometry-based proteome analysis:
Quantitative proteomics and targeted proteomics
Analysis of protein phosphorylation events
Analysis of modifications occurring on lysines
Proteomics of subcellular fractions
• Activity-based proteomics => chemical tagging and profiling of active enzymes
• Assays and screening methods for studying protein binding and enzymatic activities
• Structure-based approaches to rational drug design and molecular design
• Thermodynamic and kinetic parameters governing binding events, e.g. in protein-protein interactions, protein-ligand interactions.
• Controlling protein-protein interactions using chemical inducers and disruptors of dimerisation
• Engineering control over protein function using chemistry: how to synthesize a protein containing unnatural amino acids, labeling of proteins and peptides • Target production: overview of recombinant protein expression systems.
To acquire knowledge on how to use chemical principles and specific chemical tools for the analysis and understanding of biological systems and processes with attention for next generation drug design. The students will learn how to use their present knowledge on organic chemistry, analytical techniques, spectroscopic techniques, thermodynamics, modelling, structure of proteins, reaction mechanisms and kinetics, metabolism etc. in order to obtain a better understanding of biological processes and the interactions of biomacromolecules with ligands.
1 Knowledge on the possibilities of proteomics technologies for the analysis of chemical biology research questions
2 Insight in chemical application of post-genomic information.
3 Knowledge on the design and use of markers and labels for the visualisation of biological processes.
4 Knowledge of several biophysical methods for the study of biochemical interactions involving small molecules interacting with biomolecules.
5 Knowledge on the chemical regulation of biosynthetic pathways.
6 Knowledge on the current methods for synthetic nucleic acid and protein production and production hosts.
7 Knowledge on the different methods for protein synthesis and modification.
8 Knowledge on chemical tools for studying protein structure and function.
9 Knowledge on methods for studying and controlling protein protein interactions.
10 Knowledge on chemical ligation methodologies.
De beoordeling bestaat uit volgende opdrachtcategorieën:
Examen Andere bepaalt 100% van het eindcijfer
Binnen de categorie Examen Andere dient men volgende opdrachten af te werken:
periodic evaluation
Deze aanbieding maakt deel uit van de volgende studieplannen:
Master of Chemistry: Chemical Theory, (Bio)Molecular Design and Synthesis (enkel aangeboden in het Engels)