4 ECTS credits
117 h study time
Offer 1 with catalog number 1022056BNR for all students in the 2nd semester at a (B) Bachelor - advanced level.
The practica are compulsory for all students.
The basic conditions of cell and tissue cultures, and celfractionation techniques are reviewed. This is followed by an overview of the current methods to separate subcellular particles and bio-molecules (i.e. filtration, dialysis, centrifugation, adsorption and liquid/gas-chromatography, electrophoresis). Finally, the most versatile detection and quantitative techniques (radio-isotopes, spectroscopy and colorimetry, quantitative PCR, immunological techniques and biosensors) are worked out.
The classes and practicals are given by Prof. S. Eeltink (60%) and Dr. B. Stijlemans (40%). Practicals are compulsory for all students.
Book: Analytical Biochemistry, authors: David J Holme & Hazel Peck, Pearson Education Ltd. (ISBN 0-582 29438-X)
Book: Bioanalytical Chemistry, Imperial College press (ISBN 1-86094-371-3)
Notes of course and powerpointpresentation shown during course are available on the learning platform.
additional information
Book: F. F. Robyt en B. J. White - Biochemical techniques Theory and Practice, Brooks/Cole Publishing Company (ISBN 0-534-07944-X)
The student knows the general principles underlying the separation, detection and dosing of biochemical molecules.
The student is capable to apply the proper separation, dosing and detection methods of biochemical molecules.
The student is capable to evaluate the pros and cons of the various chromatograf-phic separation methods.
The final grade is composed based on the following categories:
Written Exam determines 100% of the final mark.
Within the Written Exam category, the following assignments need to be completed:
The final mark is composed of:
- 80% of the final mark is based on a written exam (60% of the points is based on open questions; 40% of the evaluation is based on multiple choice questions). The question will cover the complete course.
- 20% of the final mark is based on the practical courses. (50% of the points is based on the evaluation of the lab report; 50% of the points is based on effort). Students are required to attend the lab sessions.
Example of exam questions:
- Open question: What are the different types of centrifugation en explain the operating principles?
- Multiple choice question: If you would like to detect a glycoprotein via blotting, what detection approach would you use? (a) Western blotting; (b) Northern blotting; (c) Southern blotting.
This offer is part of the following study plans:
Bachelor of Bioengineering Sciences: Profile Cell and Gene Biotechnology (only offered in Dutch)
Bachelor of Bioengineering Sciences: Profile Chemistry and Bioprocess Technology (only offered in Dutch)
Bachelor of Bioengineering Sciences: Initial track (only offered in Dutch)